Table 6.
Effect of Test Agents on Cocaine-Induced Inhibition of [3H]DA Uptake by Striatal Synaptosomes
| Drug | IC50 (nM±SD) | Specific Uptake1 (% of Control±SD) |
|---|---|---|
| Cocaine (n=6, 60 data points) | 278±9 | 100 |
| Cocaine+SoRI-20040 (12.8 μM) (n=3, 30 data points) | 402±30* | 43±10 |
| Cocaine+SoRI-20041 (12.8 μM) (n=3, 30 data points) | 450±20* | 27±4 |
| Cocaine+SoRI-2827 (12.8 μM) (n=3, 30 data points) | 582±45* | 44±6 |
| Cocaine+Indatraline (7 nM) (n=3, 30 data points) | 894±100* | 31±7 |
Ten point cocaine inhibition curves (50 - 5052 nM) were generated using 2 nM [3H]DA in the absence and presence of the indicated test agents. The data of several independent experiments were pooled and fit to the two parameter logistic equation for the best-fit estimate of the IC50 (±SD).
1
Each test agent reduced specific [3H]DA uptake and the degree of reduction is reported as a percent of control.
*
p<0.05 when compared to the IC50 of cocaine (Students t-test).