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Screen of slm mutants. The cdc25–22 strain (KS1483) transformed with p25SS carrying cdc25+ and ura4+ was mutagenized and grown on YES (glucose and yeast extract with supplements) agar medium at 25°C. Nonsynthetic lethal mutants can lose p25SS and remain viable because YES medium contains uracil (left panel). On the other hand, synthetic lethal mutants undergo cell cycle arrest after they lose p25SS (indicated by arrowheads in the right panel).
