Figure 7.

(A) Wild-type (PR109), Δcdr2 (JK2240), cdc2–3w (JM300), Δcdr2 cdc2–3w (JK2258), cdc2–3W Δcdc25 (GL192) and Δcdr2 cdc2–3W Δcdc25 (JK2259) strains were grown in YES media at 30°C. Photographs were taken using differential interference contrast optics. Bar, 10 μm. Numbers in panels refer to cell length at division. (B) cdr2 deletion does not cause delay of mitosis after inactivation of Wee1 and Mik1 kinases. Synchronous cultures of Δmik1 wee1–50 (AB90) or Δcdr2 Δmik1 wee1–50 (JK2244) strains in early G2 phase were grown in YES media at 25°C and then shifted to 35°C to inactivate Wee1–50 protein. The cultures were examined microscopically to determine the percentage of the cells that had attempted mitosis.