Table 1.
S. pombe strains used in this study (all strains are leu1-32 ura4-D18)
| Strains | Genotype | Source or reference |
|---|---|---|
| AB90 | h− mik1::LEU2+ura4+ wee1-50 | Lab stock |
| PR109 | h− | Lab stock |
| GL192 | h+ cdc2-3w cdc25::ura4+ | Lab stock |
| JM298 | h− wee1-50 | Lab stock |
| JM300 | h− cdc2-3w | Lab stock |
| PR1319 | h+ ade6-210 his7-366 | Lab stock |
| PR1320 | h− ade6-216 his7-366 | Lab stock |
| KS1361 | h− cdc25-22 wee1-50 his3-237 | Lab stock |
| KS1483 | h+ cdc25-22 | Lab stock |
| LW1817 | h− nim1::ura4+ | Lab stock |
| JK1864 | h− cdc25-22 | This study |
| JK2240 | h− cdr2::ura4+ | This study |
| JK2241 | h− cdr2::ura4+ nim1::ura4+ | This study |
| JK2242 | h− cdr2::ura4+ wee1-50 | This study |
| JK2243 | h− cdr2::ura4+ cdc25-22 wee1-50 | This study |
| JK2244 | h− cdr2::ura4+ mik1::LEU2 wee1-50 | This study |
| JK2246 | h− slm9::ura4+ | This study |
| JK2248 | h− slm9::ura4+ cdc25-22 wee1-50 | This study |
| JK2249 | h+ cdr2-1 | This study |
| JK2258 | h− cdr2::ura4+ cdc2-3w | This study |
| JK2259 | h− cdr2::ura4+ cdc2-3w cdc25::ura4+ | This study |
| JK2310 | h− cdr2HA6H(ura4+) | This study |
| JK2312 | h− cdr2HA6H(ura4+) cdc25-22 | This study |