Table 3.

GO classifications altered by rotenone.

GO Number	Name	UP	DOWN	p-value	CLASS
GO:0008150	biological process	436	458
Categories increased by rotenone
GO:0009117	nucleotide metabolism	15	4	0.010	Met
GO:0006091	generation of precursor metabolites and energy	39	8	<0.001	Met
GO:0006118	electron transport	27	6	<0.001	Met
GO:0006066	alcohol metabolism	22	3	<0.001	Met
GO:0006694	steroid biosynthesis	9	1	0.010
GO:0016126	sterol biosynthesis	7	0	0.006
GO:0016072	rRNA metabolism	9	0	0.001	Prot
GO:0006364	rRNA processing	8	0	0.003	Prot
Categories decreased by rotenone
GO:0030001	metal ion transport	0	13	<0.001
GO:0009966	regulation of signal transduction	4	18	0.004
GO:0003674	molecular function	452	489
Categories increased by rotenone
GO:0016491	oxidoreductase activity	61	18	<0.001	Met
GO:0009055	electron carrier activity	22	7	0.002	Met
GO:0016651	oxidoreductase activity, acting on NADH or NADPH	14	1	<0.001	Met
GO:0043167	hydrogen ion transporter activity	13	1	0.001	Met
GO:0003954	NADH dehydrogenase activity	12	1	0.001	Met
GO:0004298	threonine endopeptidase activity	7	0	0.006	Prot
GO:0015077	structural constituent of ribosome	13	2	0.003	Prot
Categories decreased by rotenone
GO:0004871	metal ion binding	93	145	0.002
GO:0008270	cation binding	77	129	0.001
GO:0046872	zinc ion binding	49	88	0.002
GO:0004872	signal transducer activity	23	50	0.003
GO:0004888	receptor activity	14	37	0.002
GO:0004888	transmembrane receptor activity	5	21	0.003
GO:0005575	cellular component	422	464
Categories increased by rotenone
GO:0044428	nuclear part	53	33	0.006
GO:0000228	nuclear chromosome	8	0	0.003
GO:0005739	mitochondrion	66	13	<0.001	Met
GO:0031966	mitochondrial membrane	11	1	0.002	Met
GO:0005743	mitochondrial inner membrane	10	1	0.004	Met
GO:0005759	mitochondrial matrix	9	0	0.001	Met
GO:0005761	mitochondrial ribosome	7	0	0.005	Met/Prot
GO:0000502	proteasome complex	10	1	0.004	Prot
GO:0005839	proteasome core complex	7	0	0.005	Prot
Categories decreased by rotenone
GO:0005783	endoplasmic reticulum	16	41	0.002	Prot

UP and DOWN columns indicate the number of transcripts altered by rotenone in a particular GO classification under each main GO hierarchy. Only statistically significant classifications (p ≤ 0.01, Fisher's exact test) are listed. Redundant or uninformaitve classifications were eliminated. Two overaching themes (CLASS) were noted in the data: Metabolism and Protein handling.