Table 2.

Albu-CocH Rescues from Cocaine Overdose

Sex	Females				Males
Enzyme dose	Albu-CocH 3 mg/kg		Albu-CocH 10 mg/kg		Albu-CocH 3 mg/kg		Albu-CocH 10 mg/kg		WT 3 mg/kg
Number of rats	5		3		3		3		3
Time window (min)	1–20	20–60	1–20	20–60	1–20	20–60	1–20	20–60	1–20
	Percent of subjects displaying specific toxic signs
Pupils +	100	100	100	100	100	100	100	100	100
Pupils ++	100	100	100	33	100	67	33	0	100
Pupils +++	100	100	100	0	100	0	0	0	100
Piloerection	100	20	0	0	33	0	0	0	100
Hyperlocomotion	60	0	67	0	67	0	0	0	a
Hard breathing	100	0	100	0	100	0	67	0	100
Head bobbing	0	0	0	0	100	0	0	0	a
Ataxia	40	0	0	0	33	0	0	0	100
Prostration	20	0	0	0	33	0	0	0	100
Seizure	20	0	0	0	0	0	0	0	100
Recovery		80		100		100		100	0

A total of 17 rats received cocaine, 100 mg/kg, i.p. Enzyme treatments in the indicated doses were administered i.v. immediately after full-blown clonic-tonic seizures had developed. Signs of toxicity were then observed at frequent intervals until the animals made a full recovery, died, or required euthanasia. Observations are summarized separately for the time windows from 1–20 min and 20–60 min after rescue treatment. Statistical significance of rescue effects was tested by χ². Because no sex differences were apparent, data from enzyme-rescued rats were compared with pooled data from the 15 males and females that received no Albu-CocH either before or after the cocaine challenge. Bold figures represent significance, with p<0.01 when treated males and females were pooled, and p<0.05 when they were separately compared with the pooled controls.

^aRats given wild-type BChE remained moribund and did not show hyperlocomotion or head bobbing.