Skip to main content
. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: J Invest Dermatol. 2008 Apr 10;128(9):2334–2342. doi: 10.1038/jid.2008.77

Figure 1. Cytokine-induced activation of proMMP-9 in human skin is inhibited by α-ACT but not by α-AT.

Figure 1

Explants of full thickness normal human skin donors were cultured in DMEM and treated with TGF-β and TNF-α. Purified human α-ACT and α-AT were added to the skin culture at the indicated concentration. After culture for 3 days, the conditioned medium was resolved by gelatinolytic zymography. The identity of MMP-9 and MMP-2 has been previously confirmed by western blot.