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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: J Invest Dermatol. 2008 Apr 10;128(9):2334–2342. doi: 10.1038/jid.2008.77

Figure 3. A conserved leucine (P1) in the RCL of α-ACT is essential for inhibition of proMMP-9 activation.

Figure 3

(a) Sequence information of the RCL of human α-ACT indicates the P1 leucine and the variants. (b and c) The α-ACT variants were expressed by IKCs transduced by lentivirus. A polyhistidine tag was added to the C terminus of the α-ACT variants. The α-ACT variants were purified from the conditioned medium by Ni-NTA column and examined by Coomassie blue and western blot analysis. (d and e) Inhibition by the α-ACT variants was assayed by incubation with proMMP-9 and skin extract, and the reactions were revealed by zymography.