Table 2.

Membrane properties of isolated control and GRO/KC treated neurons in whole cell current clamp experiments

Cell and AP Properties	IB4-negative cells		IB4-positive cells
	Control	GRO/KC	Control	GRO/KC
Number of Cells	26	22	25	18
Diameter, μm	20.0 ± 0.8	21.2 ± 0.7	22.8 ± 0.5	20.2 ± 0.9*
Cell Capacitance, pF	17.6 ± 1.3	20.0 ± 1.2	19.0 ± 1.3	16.8 ± 1.7*
Resting Potential, mV	-63.0 ± 1.6	-56.0 ± 1.4**	-64.6 ± 1.4	-55.4 ± 1.9 **
Input Resistance, MΩ	1244.0 ± 144.3	986.1 ± 99.4	1062.0 ± 64.6	1427.4 ± 92.3**
AP Rheobase, pA	60.4 ± 8.4	21.1 ± 2.1**	76.2 ± 14.2	51.1 ± 11.2*
AP Threshold, mV	-19.7 ± 0.9	-28.6 ± 1.4**	-16.6 ± 0.6	-25.4 ± 1.3**
AP Rising Rate, mV/ms	106.8 ± 9.6	112.5 ± 10.7	103.4 ± 8.8	102.4 ± 12.0
AP Falling Rate, mV/ms	49.7 ± 4.8	61.9 ± 7.3	43.4 ± 3.8	39.9 ± 5.0**
AP Amplitude, mV	64.8 ± 1.7	74.8 ± 1.8**	63.1 ± 1.9	71.1 ± 1.5 **
AP Duration, ms	6.6 ± 0.4	7.3 ± 0.5	8.3 ± 0.5	14.0 ± 1.0**
AHP Amplitude, mV	3.5 ± 0.5	5.9 ± 0.6 **	3.0 ± 0.8	4.5 ± 0.7
AHP Duration, ms	10.8 ± 2.4	14.7 ± 0.8*	7.7 ± 1.7	9.9 ± 1.4

Values are mean ± SEM. AP, action potential; AHP, afterhyperpolarization. * P < 0.05, ** P < 0.01, comparing GRO/KC treated vs. Control cells with the same IB4 status (Student's t-test or Mann-Whitney test). Rheobase is defined as the smallest depolarizing current to elicit at least one action potential within 80 msec. Action potential parameters were measured on this first elicited action potential.