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Titration of amantadine against influenza A and B viruses in the AVINA assay. Cells were infected with A/PR/8/34, A/Memphis/14/98 and B/Jiangsu/10/2003 at 0.01 MOI in the presence of serial dilutions of amantadine. The next day, NA activity was measured in the supernatant and IC50 calculated by GraphPad Prism software.
