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Titration of bis-indolyl maleimide (BIM) against A/Memphis/14/98 in the AVINA assay. Cells were infected with 0.01 MOI A/Memphis/14/98 in the presence of serial dilutions of BIM. The next day, NA activity was measured in the supernatant (shown as relative fluorescence units) and IC50 calculated by GraphPad Prism software. Cell viability was determined by ATPlite assay (shown as relative light units).
