Figure 7.

Identification of amantadine-resistant virus preparations. A/Memphis/14/98 was cultured in the presence of amantadine for one passage (A/Mem/98 p1), two passages (A/Mem/98 p2), or 4 passages (A/Mem/98 p4) in tissue culture. The TCID₅₀ of each virus passage was determined, and 0.02 MOI added to serial dilutions of amantadine in the AVINA assay. The percent inhibition of virus replication is shown at each concentration of amantadine was calculated by: 100 × (average RFU at each amantadine concentration/average RFU in the absence of amantadine).