Figure 3.

α2β1-mediated K562 transfectant cell migration on collagen and laminin upon stimulation by JBS2. Migration of KX2C2 (A and B) and KX2C2(I⁻) (C and D) transfectant cells were carried out on polycarbonate filters (10-μm pore size) coated with collagen (A and C) or laminin (B and D) at 10 μg/ml in the presence of α2β1 stimulatory mAb JBS2 or in combination with 10 mM Mn²⁺. The role of α2β1 was determined using blocking mAb BHA2.1, with the isotype matched mAb P3 as control. All mAbs were used at 20 μg/ml. Values were enumerated from the average (±SD) of cell counts from five random high-power fields (400×) from a minimum of three independent experiments.