Figure 4.

Determination of adhesive strength of KX2C2 cells bound onto collagen. KX2C2 cells were labeled with BCECF fluorescence label, treated with various concentrations of NmIg, JBS2, or JBS2 with Mn²⁺ at 20 μg/ml, and allowed to adhere to collagen-coated wells for 45 min at 37°C. Nonadherent cells were removed by centrifugation at 17 × g (A) or 30 × g (B). Adherent cells were expressed as the percentage of bound cells based on the fluorescence of 5 × 10⁴ labeled cells after a subtraction of background fluorescence.