Figure 6.

Determination of adhesive strength of RDX2C2 cells bound onto collagen. RDX2C2 cells were labeled with BCECF fluorescence label, treated with various concentrations of mAb P3, BHA2.1, or BHA2.1 with JBS2, at a constant concentration of 20 μg/ml, and allowed to adhere to collagen-coated wells for 45 min at 37°C. Nonadherent cells were removed by centrifugation at 17 × g (A) or 30 × g (B). Cell adhesion was expressed as the percentage of bound cells based on the fluorescence of 5 × 10⁴ labeled cells after a subtraction of background fluorescence.