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. 2008 Oct 24;4(10):e1000188. doi: 10.1371/journal.ppat.1000188

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Pomel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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To ensure that the observed redistribution of aldolase-1 is not a fixation artifact we also compared the distribution of aldolase-1 and cytoplasmic YFP in extracellular (EC) and intracellular (IC) Toxoplasma fixed in methanol (MeOH) or 3% paraformaldehyde/0.01% glutaraldehyde (PFA/GAH). Merged images of the various proteins and the parasite nuclei are shown as well as DIC images. Bars = 2 µm.