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. 2008 Oct 24;4(10):e1000188. doi: 10.1371/journal.ppat.1000188

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Pomel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Freshly egressed parasites expressing myc-aldolase 1 were allowed to attach to HFF monolayers for 5 minutes at 37°C in complete medium. Non-attached parasites were washed off and the incubation continued in complete medium at 37°C for the indicated times. (A) The distribution of aldolase-1 30 minutes and 4 hours after invasion of host cells. Overlays are shown of myc-aldolase-1 (mALD1, green), the IMC protein IMC1 (red). Overlay pictures of DIC and DAPI blue) are shown on the right. Parasite-infected cells were fixed in −20°C methanol. Images of the individual channels are shown in Figure S4. Bars = 2 µm. (B) A quantitative analysis of the results is shown. Data represent averages of 32 fields counted over 4 samples. Error bars indicate the standard deviation.