Figure 8. Redistribution of other glycolytic enzymes to the pellicle of extracellular parasites.

The subcellular location of myc-tagged hexokinase (mHK), glyceraldehyde-3-phosphate dehydrogenase-1 (mGAPDH1), pyruvate kinase-1 (mPK1), or lactate dehydrogenase-1 (mLDH1) was determined in (A) intracellular and (B) extracellular Toxoplasma tachyzoites by immunofluorescence microscopy after fixation in −20°C methanol. The IMC of the parasites was visualized using antibodies to IMC1 (red) and their nuclei using DAPI (blue). The separate images are shown in Figure S5. Bars = 2 µm.