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. 2008 Oct 17;4(10):e1000227. doi: 10.1371/journal.pgen.1000227

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Sancho et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Nuclei from H1 variant knock-down cells treated or not with Dox for 6 days were treated with MNase and the profile of bulk chromatin was analyzed in gel electrophoresis to calculate the nucleosome repeat length (NRL). (B) Plot of nucleosome number versus DNA length for MNase-digested bulk chromatin of H1.2 knocked-down cells ±Dox. This plot has been used to calculate the corresponding NRL. Data from 5 independent experiments has been pooled. Average±SD is shown. Data from untreated and treated samples differed significantly in a t-Student test (95% confidence interval with a p value of 0.0001). Open triangles denote untreated, and solid square symbols correspond to cells treated for 6 days with Dox. (C) Complementation of reduced nucleosome spacing in H1.2 knocked-down cells by stable expression of shRNA-resistant H1.2 (rH1.2HA).