Figure 1. Steady state hydrogen peroxide levels are elevated in CF in the absence and presence of inflammatory stimulation.

H₂O₂ levels were assayed in: A) Two immortalized cell line pair models of CF, the 16 HBEo− (sense and antisense (AS)) and 9 HTEo− (pCEP and pCEP-R), and; B) one polarized primary cell model, the wd-HPTE (untreated or treated with 20 µM CFTR_inh-172 for 72 hrs.). Unstimulated cells are compared to cells harvested 24 hrs following 1 hr. incubation with TNF-α/IL-1β (10 ng/ml each). * connotes significant difference from unstimulated normal control (p<0.05), while ** connotes significant difference from both unstimulated and stimulated normal control. Each data bar represents the average of 8 replicate wells in 4 experiments for (A), or 3 replicate wells from donors 1 and 2, or 2 replicate wells for donor 3 in 1 experiment for (B).