Figure 8. Schematic of the mechanism of H₂O₂ regulation its impact on inflammation in CF.

Our proteomic analysis of in vitro and in vivo models of CF indicate differential expression of redox proteins that regulate H₂O₂, predicting an increase in peroxide, which we confirmed by biochemical analysis. The mechanism involves a paradoxical decrease in Nrf-2 expression and activity caused by the loss of CFTR function in CF cells. Furthermore, we demonstrate the effect of this phenomenon on inflammation in CF.