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. 2008 Oct 1;105(40):15581–15586. doi: 10.1073/pnas.0800621105

Fig. 4.

Fig. 4.

Increase of mEPSC and mIPSC frequency in synaptotagmin 1-deficient mice. (A) Overlay of 20 consecutive original traces of mEPSCs recorded in a BC at −70 mV under control conditions in a wild-type (upper traces) and a synaptotagmin 1-deficient mouse (lower traces). Inset shows single representative mEPSC from wild-type culture (Scale bars: 50 pA, 5 ms.) (B) Cumulative histograms of mEPSC interevent interval (black curve, wild-type; red curve, knockout). Data in A and B were obtained from the same cells. (C) Summary bar graph for mean frequency and peak amplitude of mEPSCs in wild-type (open circles) and knockout (filled circles) mice. Data are from 6 and 10 BCs, respectively. Bars represent mean values, circles show data from individual experiments. In all experiments, 1 μM TTX and 10 μM bicuculline were added to the bath solution. (D–F) Original traces of mIPSCs recorded in a GC at −80 mV (D), histogram of interevent interval and peak amplitude (E), and summary bar graphs for mean frequency and peak amplitude of mIPSCs (F). Data from 7 and 8 GCs, respectively. Inset in D shows single representative mIPSC from wild-type culture (Scale bars: 50 pA, 20 ms). In all experiments, 1 μM TTX, 10 μM CNQX, and 20 μM D-AP5 were added to the bath solution.