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EVV 2DIR spectra of pepsin measured with two different polarization combinations: PPP (all beams having their fields in the plane of propagation) and PPS (IR beams polarized in the plane of propagation and the visible normal to the IRs). The spectra were measured for the same set of pulse delays: T12 = 2 ps, T23 = 1 ps, and are plotted on the same intensity scale. The cross-peaks used in this study were mainly identified from previous studies of peptides (12).
