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Characterization of HS-SLAC. (A) In-gel activity assay of SLAC and HS-SLAC in a 16% tricene native gel. SLAC washed with 30 mM DMP (lane 1) and Coomassie blue stained (lane 2). HS-SLAC washed with 30 mM DMP (lane 3), and Coomassie blue stained (lane 4). (B) Dilute solution activity of HS-SLAC with DMP and air-saturated solution, 10 mM NaiP, pH 7.0, at 25°C. (C) CD spectra of SLAC (dots) and HS-SLAC (solid) at pH 7.0.
