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. 2008 Oct 7;105(40):15547–15552. doi: 10.1073/pnas.0805203105

Fig. 1.

Fig. 1.

Two cannibalism operons are under the indirect control of AbbA. (A and B) AbbA is required for maximal expression of the sdpABC and skfABCDE operons. (A) Strains harbored amyE::Psdp-lacZ, and were either wild-type (♦; RL4264), or mutant for abbA (●; AB149). (B) Strains harbored amyE::Pskf-lacZ, and either were wild-type (♦; RL3554), or mutant for abbA (●; AB148). (C and D) An abrB mutation is epistatic to the effect of an abbA mutation on expression of the sdpABC and skfABCDE operons. (C) Strains harbored amyE::Psdp-lacZ, and were wild-type (♦; RL4264), mutant for abbA (●; AB149), harbored the overexpression construct Phyperspank-abbA (○; AB151), mutant for abrB (■; AB183), mutant for both abbA and abrB (▴; AB184), or mutant for abrB and harbored Phyperspank-abbA (Δ; AB182). (D) Strains harbored Pskf-lacZ, and were either wild-type (♦; RL3554), mutant for abbA (●; AB148), harbored Phyperspank-abbA (○; AB150), mutant for abrB (■; AB188), mutant for both abbA and abrB (▴; AB189), or mutant for abrB and harbored Phyperspank-abbA (Δ; AB190). Cells were grown in liquid DS medium; hour 0 of sporulation was the end of the exponential phase of growth. Expression of Phyperspank-abbA was induced by the addition of 1 mM (final concentration) IPTG to the medium.