FIG. 4.

Effects of various doses of PFDA on Oatp1a1, 1a4, and 1b2, as well as Ntcp mRNA expression in mouse livers. Adult male C57BL/6 WT mice were administered PFDA at a single i.p. dose (0.5, 1.0, 10, 20, 40, or 80 mg/kg of body weight), whereas control mice were treated i.p. with the vehicle, propylene glycerol:water (1:1, vol/vol). After 2 days, livers were collected. Total RNA samples from treated male mouse livers (n = 4) were analyzed by the bDNA assay. The data are reported as RLUs per 10 μg of total RNA, and are presented as mean ± SEM. Asterisks indicate statistically significant differences between control and PFDA-treated male mice (p < 0.05).