FIG. 7.

Effects of PFDA on mouse Oatp1a1, 1a4, and 1b2, as well as Ntcp mRNA expression in wild-type and PXR-null mice. The mouse treatments are separated into four groups: PXR^(+/+) + CONT, wild-type mice treated with the vehicle, propylene glycerol:water (1:1, vol/vol); PXR^(+/+) + PFDA, wild-type mice treated with PFDA at dose 80 mg/kg of body weight; PXR^(−/−) + CONT, PXR-null mice treated with the vehicle; PXR^(−/−) + PFDA, PXR-null mice treated with PFDA at dose 80 mg/kg. The mice were given a single i.p. administration. After 2 days, livers were removed. Total RNA from untreated or treated mouse livers was analyzed by the bDNA assay. All data are expressed as mean ± SEM of five mice for each treatment. Asterisk indicates statistically significant difference between PFDA-treated and control mice (p < 0.05); single dagger (†) represents statistically significant differences (p < 0.05) between the vehicle-treated wild-type male mice and the vehicle-treated PXR-null male mice.