FIG. 9.

Effects of PFDA on Oatp1a1, 1a4, and 1b2, as well as Ntcp mRNA expression in wild-type and FXR-null mice. The black and the dark-latticed bars represent regulation of each transporter by vehicle treatment; the striated bars depict regulation of each transporter by PFDA treatment. Adult C57BL/6 wild-type or FXR-null male mice were treated with the vehicle, propylene glycerol:water (1:1, vol/vol) or PFDA at dose 80 mg/kg of body weight. The mice were treated for 2 days. Total RNA from untreated or treated mouse livers was analyzed by the bDNA assay. All data are expressed as mean ± SEM of five mice for each treatment. Asterisk indicates statistically significant difference between PFDA-treated and control mice (p < 0.05). Single dagger (†) represents statistically significant differences (p < 0.05) between the vehicle-treated wild-type male mice and the vehicle-treated FXR-null male mice.