Fig. 2.

DNA fragmentation induced by 7-ketocholesterol (7KC), but not staurosporine, is diminished by CB2 deficiency in macrophages. A: In situ TUNEL analysis of CB2^+/+ and CB2^−/− MPMs after 16 h in the presence of increasing concentrations of 7-ketocholesterol (7KC) as indicated. The MPMs were also treated with 50 μg/ml OxLDL as a positive control. B: TUNEL analysis of CB2^+/+ and CB2^−/− MPMs incubated for 6 h in the presence or absence of 1 μM staurosporine. The graph shows the mean percentage ± SD of TUNEL stained nuclei observed for each treatment performed in triplicate for two independent experiments. * P < 0.001 between similarly treated CB2^−/− and CB2^+/+ macrophages.