Table 1 SEMA4A gene primers and PCR conditions.

Exon		Primer sequence (5′→3′)		PCR product size (bp)		MgCl2, (mmol/l)		Temp (°C)
1F		CCACCAACTTCCGCCCAAGCC		372		1.5		60
1R		CCGGCTCCCAGCCTCTGCAGC
2F		CCTGCCACCAATACACACGC		389		1.5		60
2R		TGTCCCTGTTTCTCCATATCC
3F		TGCATCTAGCTGCCAAGGCC		375		1.5		67
3R		TTATTCATGCACAGGCAGCC
4F		ATCAGCATGTCACTAACCACC		320		1.5		57
4R		AACATAATCCAGGAGATAACC
5F		AGCATTACCTAGCCTTTCTCC		299		1.5		58
5R		CATCTGGAAGGCCAGAGTTTTCC
6F		AGACCAATTTCCCTCTATGTCCC		368		1.5		59
6R		AGCATCCTTCAACTTCAGCTTCC
7F		GGTGCAGAACTGATGCAGGG		402		1.5		60
7R		GAAAGCTTCAAGAGAACCGGAG
8F		GAGGAAGCCTGTGTGTCCTGG		283		1.5		62
8R		TATTGGTTCTCCTCCAGTTAG
9F		GGGTGGCTAGAGTCTGCAAGG		623		1.5		60
9R		GAAAGTGCTGAGTAGGTGCTGG
10F		CTCCCTTGGCCCTTATCAACAC		409		1.5		60
10R		GGCATAGTAAATGCCCAATAAA
11F		CCACCCTGAAATGAGGACTGCC		434		1.5		65
11R		CCAGGCACATAGCAGGTGCTC
12F		CCGCGCCCAGTACATATCACCC		335		1.5		68
12R		CCCAGCACGCTGGTGCAGGCC
13F		CCTGACCATCAAATGGCCTTCC		441		1.5		60
13R		CCATCCCTGGAGGAACCCACTGC
14F		TTCCGCGTTCCTCTCTCTGTTCC		361		1.5		55
14R		TCGGGTGTTCAGATCTCAAGCC
15.1F		GGGCTGGGTCCAAAGATAGG		437		1.5		71
15.1R		GACTGCTGGGCAGCCAGGGCG
15.2F		CTTTTCATACCCTGTGATCTCC		531		2.5		65
15.2R		CAGAGTAGCAGAAGCTCCTCTC

F, Forward; R, Reverse.