Figure 8 Resorption inhibition in control osteoclasts. Blood derived differentiated osteoclasts were cultured on bone slices for a week in the presence of the indicated concentrations of bafilomycin A1, EIPA, or both, either in neutral (pH 7.25) or acidic (pH 6.90) media. Bone resorption was then quantified as pit indices. (A) Concentration dependent decrease of resorption in bafilomycin treated cultures. (B) Concentration dependent decrease of resorption in EIPA treated cultures. (C) Quantification of resorption at maximum doses used. No detectable resorption was noted in cultures treated with both inhibitors. (D) TRAcP staining of osteoclast cultures treated with vehicle or bafilomycin A1, EIPA, or both. No evident alterations in cell morphology were noted. Baf, bafilomycin A1.