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. 2005 Jul 13;43(3):225–231. doi: 10.1136/jmg.2005.034058

Table 1 Primers used for sequencing of GDF5.

Exon Forward Reverse DNA Polymerase Annealing temp (°C) Product length (bp)
1 CAGCATTACGCCATTCTTCCT CCGGTGCCTCCCTTTG Platinum Taq 63 613
1 GGGACCAGGCCAGGATTG GGGCCAGATGCACACAGTG Platinum Taq 63 637
2 GACTGGCTCCCTTGGT GGAATGCGGTTAGAGGTGAGC Platinum Taq 63 1688
2 ATGAGATTAAGGCCCGCTCTG TGTCTCCCTGGACCTGTGC Platinum Taq 60 692
2 GGAGGCGGGCGGGCTGCCCA GCAGAGTCAATGAAGAGGAT NEB Taq 55 680
2 GGCCCCCTTTTATCCACAAGT* GTCGAACACCTCCCAGCCAG*

*Sequencing primer.