Table 1 Primers used for sequencing of GDF5.
Exon | Forward | Reverse | DNA Polymerase | Annealing temp (°C) | Product length (bp) |
---|---|---|---|---|---|
1 | CAGCATTACGCCATTCTTCCT | CCGGTGCCTCCCTTTG | Platinum Taq | 63 | 613 |
1 | GGGACCAGGCCAGGATTG | GGGCCAGATGCACACAGTG | Platinum Taq | 63 | 637 |
2 | GACTGGCTCCCTTGGT | GGAATGCGGTTAGAGGTGAGC | Platinum Taq | 63 | 1688 |
2 | ATGAGATTAAGGCCCGCTCTG | TGTCTCCCTGGACCTGTGC | Platinum Taq | 60 | 692 |
2 | GGAGGCGGGCGGGCTGCCCA | GCAGAGTCAATGAAGAGGAT | NEB Taq | 55 | 680 |
2 | GGCCCCCTTTTATCCACAAGT* | GTCGAACACCTCCCAGCCAG* |
*Sequencing primer.