Table 1.
Yeast strains
| Strain | Relevant genotype | Source |
|---|---|---|
| LH103 | MATa mec1-1 mad1Δ::HIS3 clb2::CLB2-LacZ bar1 | This work |
| YCp50/MAD1 | ||
| AFS34 | MATa ade2-1 can1-100 ura3-1 leu2-3,112 his3-11,15 trp1-1 | R. Rothstein |
| (W303-1a) | ||
| YPH218 | MATα cdc16-1 | P. Hieter |
| YPH221 | MATα cdc23-1 | P. Hieter |
| LH127 | MATα cdc20-1 ura3-1 leu2-3,112 trp1-1 | This work |
| H160-3-3 | MATα cdc27-1 | L. Hartwell |
| H152-4-2 | MATα cdc26-1 | L. Hartwell |
| ELW65-93 | MATa cdc31-2 YCp50/CDC31 | M. Winey |
| CMY763 | MATα cim3-1 | C. Mann |
| CMY765 | MATα cim5-1 | C. Mann |
| LH202 | MATa cim5-1 bar1 | This work |
| LH103-15 | MATa doc1-1 mec101 mad1Δ::HIS3 clb2::CLB2-lacZ bar1 | This work |
| YCp50/MAD1 | ||
| LH103-66 | MATa cdc26-100 mec1-1 mad1Δ::HIS3 clb2::CLB2-lacZ bar1 | This work |
| YCp50/MAD1 | ||
| LH225 | MATa cdc26-100 clb2::CLB2-lacZ bar1 | This work |
| LH226 | MATa doc1-1 clb2::CLB2-lacZ bar1 | This work |
| ADR58 | MATa pDK27 (GAL-CLB2) | This work |
| ADR103 | MATa cdc16-1 pDK27 | This work |
| LH227 | MATa cdc26-100 bar1 pDK27 | This work |
| LH228 | MATa doc1-1 bar1 pDK27 | This work |
| LH229 | MATa cdc26-100 bar1 ura3-1::CDC26myc-URA3 pDK27 | This work |
| LH230 | MATa doc1-1 bar1 ura3-1::DOC1myc-URA3 pDK27 | This work |
| LH209 | MATa bar1 pUb-R-βgal | This work |
| LH210 | MATa bar1 pUbV76-V-eΔK-βgal | This work |
| LH211 | MATa doc1-1 bar1 pUb-R-βgal | This work |
| LH212 | MATa doc1-1 bar1 pUbV76-V-eΔK-βgal | This work |
| LH213 | MATa cdc26-100 bar1 pUb-R-βgal | This work |
| LH214 | MATa cdc26-100 bar1 pUbV76-V-eΔK-βgal | This work |
| LH215 | MATa cim3-1 bar1 pUb-R-βgal | This work |
| LH216 | MATa cim3-1 bar1 pUbV76-V-eΔK-βgal | This work |
| LH231 | MATa bar1 pWAM10 (CDC16HA) | This work |
| LH232 | MATa bar1 pRS239 (CDC23HA) | This work |
| LH233 | MATa bar1 pJL25 (CDC27HA) | This work |
| LH234 | MATa bar1 pLH32 (DOC1HA) | This work |
| LH235 | MATa bar1 cdc26Δ::URA3 pWAM10 | This work |
| LH236 | MATa bar1 cdc26Δ::URA3 pRS239 | This work |
| LH237 | MATa bar1 cdc26Δ::URA3 pJL25 | This work |
| LH238 | MATa bar1 cdc26Δ::URA3 pLH32 | This work |
| LH297 | MATa bar1 doc1Δ::URA3 leu2-3,112::DOC13XHA-LEU2 | This work |
| pJL25 |
All strains from this work are W303 (R. Rothstein).