Table 2.
Mitotic-arrest mutants isolated by mec1-1 enrichment
| Gene | No. of alleles isolated | Biochemical function | Reference |
|---|---|---|---|
| CDC16 | 16 | APC component | Irniger et al. (1995), King et al. (1995) |
| CDC23 | 3 | APC component | Irniger et al. (1995) |
| CDC27 | 4 | APC component | King et al. (1995) |
| CDC26 | 1 | APC component | This work |
| DOC1 | 1 | APC associated | This work |
| CIM3 | 1 | Proteasome component | Ghislain et al. (1993) |
| CDC20 | 1 | Unknown | Sethi et al. (1991) |
| CDC31 | 1 | SPB component | Baum et al. (1986) |
| PRP22 | 2 | Splicing | Company et al. (1991) |
| Uncloned | 2 | Unknown |
Mutants were identified based on complementation of temperature sensitivity with previously identified mutants. They were also tested for rescue of temperature sensitivity by wild-type copies of known genes on plasmids.