Table 3.

doc1-1 arrest phenotype

	Strain	% of total
	Wild type	75	10	11	4
	doc1-1	16	1	79	4

Wild-type and doc1-1 strains were grown to logarithmic phase and then shifted to 37°C for 3 h. The cells were fixed and then stained for DNA using 4,6-diamino-2-phenylindole and tubulin by using immunofluorescence. Percentage of total cell morphologies of unbudded with a single nucleus and a G₁ microtubule array, small budded with a single nucleus and short spindle, large budded with a single nucleus and short spindle, and large budded with separated DNA masses and long spindle are indicated from left to right. Two hundred cells were counted for each strain.