Table 4.

Genetic interactions between cdc26, doc1, and other mitotic mutants

	cdc26-100	doc1-1	cdc16-1	cdc23-1	cdc27-1	cim5-1
Maximum permissive temperature (°C)	33	33	30	30	33	37
Maximum permissive temperature of double mutant with cdc26-100 (°C)	33	Dead	Dead	Dead	30	33
Maximum permissive temperature of double mutant with doc1-1 (°C)	Dead	33	23	23	30	33

Synthetic interactions among cdc26-100, doc1-1, and various mitotic mutants. cdc26-100 and doc1-1 were mated to each other or to cdc16-1, cdc23-1, cdc27-1, or cim5-1. The resultant diploids were sporulated and tetrads were dissected and germinated at 23°C. They were then tested for growth at 23°C, 30°C, 33°C, 35°C, and 37°C. The genotype of viable spores was determined by complementation testing with appropriate mutant tester strains. The maximum permissive temperatures of the cdc26-100 and doc1-1 mutants are shown in boldface type.