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. 1999 Nov;10(11):3549–3565. doi: 10.1091/mbc.10.11.3549

Figure 5.

Figure 5

Multicopy expression of SHR3 partially suppresses the temperature-sensitive phenotypes of sec12-1, sec13-1, sec13-4, and sec31-1 mutations. (A) Serial dilutions of strains transformed with pRS202 (−) and pPL250 (+) were incubated at the indicated temperatures for 3 d and photographed. Strains from left to right are PLY144 (SEC+), MAS26-1A (sec12-1), MAS35-6B (sec13-1), MAS242-7B (sec13-4), MAS203-1A (sec31-1), and MAS297-2B (sec62-1). Strains were grown on SC (minus uracil), with the exception of MAS242-7B (sec13-4), which was grown on SPD medium containing 0.6 mM histidine. (B) Multicopy expression of SHR3 suppresses the inositol requirement of sec13-1 mutations. Serial dilutions of strains transformed with pRS202 (−) and pPL250 (+) were spotted onto media without (left and center panels) and with (right panel) inositol. Culture plates were incubated at the indicated temperature for 3 d and photographed. Dilution series 1 and 2, strain MAS35-6A (SHR3 SEC13); series 3 and 4, strain MAS35-6B (SHR3 sec13-1); series 5, MAS35-3A (shr3Δ1 sec13-1).