Figure 8.

Shr3p interacts transiently with Gap1p. The expression of GST-SHR3 (pFG117) and Gap1p in strain FGY145 was induced as described in MATERIALS AND METHODS. Cultures were labeled with [³⁵S]methionine for 5 min and chased by the addition of excess unlabeled methionine and cysteine. Gap1p was immunoprecipitated from labeled extracts using anti-Gap1p antibodies directly (1^o anti-Gap1p) or in a second round of immunoprecipitation from resolubilized immunoprecipitates obtained using glutathione-Sepharose beads (1^o anti-GST and 2^o anti-Gap1p).