Figure 9.

COPII coatomer components copurify with GST-SHR3. The indicated GST fusion constructs were expressed in FGY145 and purified as in Figure 6, except that after affinity chromatography the glutathione-conjugated beads were washed four times with low-potassium FGB containing 0.02% DM (see MATERIALS AND METHODS). Proteins were denatured at 65°C for 5 min and resolved by SDS-PAGE in a 10% polyacrylamide gel. Protein bands corresponding to Sec31p, Sec24p, Sec23p, Sec13p, and Sar1p were visualized using rabbit antiserum as described in MATERIALS AND METHODS.