Table 1.
Saccharomyces cerevisiae strains
| Strain | Genotype | Source or reference |
|---|---|---|
| PLY1 | MATa ura3-52 his4Δ29 GAL+ | Ljungdahl et al., 1992 |
| FGY145 | MATa ura3-52 his4Δ29 shr3Δ6 GAL+ | This work |
| Isogenic derivatives of AA288 | ||
| AA288 | MATa ura3-52 leu2-3,112 lys2Δ201 ade2 | Antebi and Fink, 1992 |
| PLY129 | MATa ura3-52 leu2-3,112 lys2Δ201 ade2 gap1Δ∷LEU2 | Ljungdahl et al., 1992 |
| PLY144 | MATa ura3-52 ade2 lys2Δ201 | This work |
| PLY147 | MATα ura3-52 ade2 lys2Δ201 | This work |
| PLY151 | MATa ura3-52 ade2 lys2Δ201 shr3Δ1∷URA3 | This work |
| PLY155 | MATα ura3-52 ade2 lys2Δ201 shr3Δ1∷URA3 | This work |
| FGY58 | MATα ura3-52 leu2-3,112 lys2Δ201 ade2 gap1Δ∷LEU2 | This work |
| FGY60 | MATα ura3-52 leu2-3,112 lys2Δ201 ade2 gap1Δ∷LEU2 shr3Δ6 | This work |
| FGY84 | MATα ura3-52 leu2-3,112 lys2Δ201 ade2 gap1Δ∷LEU2 suc2Δ101 | This work |
| FGY85 | MATα ura3-52 leu2-3,112 lys2Δ201 ade2 gap1Δ∷LEU2 shr3Δ6 suc2Δ101 | This work |
| Original sec− mutant strains | ||
| CKY42 | MATa ura3-52 sec12-1 | Kaiser laboratory |
| CKY45 | MATα ura3-52 his4-619 sec13-1 | Kaiser laboratory |
| CKY46 | MATa ura3-52 his4-619 sec13-1 | Kaiser laboratory |
| RSY315 | MATα sec13-4 | Schekman laboratory |
| RSY316 | MATa sec13-4 | Schekman laboratory |
| CKY50 | MATα ura3-52 his4-619 sec16-2 | Kaiser laboratory |
| CKY58 | MATα ura3-52 his4-619 sec18-1 | Kaiser laboratory |
| CKY59 | MATa ura3-52 his4-619 sec18-1 | Kaiser laboratory |
| RSY281 | MATα ura3-52 his4-619 sec23-1 | Schekman laboratory |
| RSY282 | MATa ura3-52 leu2-3,112 sec23-1 | Schekman laboratory |
| RSY1004 | MATα ura3-52 leu2-3,112 sec31-1 | Schekman laboratory |
| RSY529 | MATα ura3-52 leu2-3,112 his4 sec62-1 | Schekman laboratory |
| Out-crossed sec− mutant and SEC+ control strains | ||
| MAS35-6A | MATa ura3-52 | This work |
| MAS35-6B | MATa ura3-52 sec13-1 | This work |
| MAS35-9C | MATa ura3-52 shr3Δ1∷URA3 | This work |
| MAS35-3A | MATa ura3-52 shr3Δ1∷URA3 sec13-1 | This work |
| MAS35-10B | MATa ura3-52 shr3Δ1∷URA3 sec13-1 | This work |
| MAS35-14B | MATa ura3-52 shr3Δ1∷URA3 sec13-1 | This work |
| MAS35-19D | MATa ura3-52 sec13-1 | This work |
| MAS35-1C | MATa ura3-52 shr3Δ1∷URA3 | This work |
| MAS203-1B | MATa ura3-52 lys2Δ201 | This work |
| MAS203-1A | MATα ura3-52 lys2Δ201 sec31-1 | This work |
| MAS203-6C | MATa ura3-52 lys2Δ201 shr3Δ1∷URA3 | This work |
| MAS203-12D | MATa ura3-52 lys2Δ201 shr3Δ1∷URA3 sec31-1 | This work |
| MAS203-20C | MATα ura3-52 lys2Δ201 shr3Δ1∷URA3 sec31-1 | This work |
| MAS203-1C | MATα ura3-52 lys2Δ201 ade2 shr3Δ1∷URA3 sec31-1 | This work |
| MAS202-3C | MATα ura3-52 lys2Δ201 sec31-1 | This work |
| MAS203-5B | MATa ura3-52 lys2Δ201 shr3Δ1∷URA3 | This work |
| MAS26-1A | MATa ura3-52 sec12-1 | This work |
| MAS242-7B | MATα ura3-52 sec13-4 | This work |
| MAS296-1A | MATα ura3-52 sec16-2 | This work |
| MAS21-1C | MATα ura3-52 sec18-1 | This work |
| MAS226-3C | MATα ura3-52 sec23-1 | This work |
| MAS297-2B | MATa ura3-52 sec62-1 | This work |