Table 2.
Plasmids
| Plasmids | Description | Source or reference |
|---|---|---|
| pPL247 | 3.5-kb SpeI–SalI fragment containing GAP1 in pRS316 | Ljungdahl et al., 1992 |
| pPL250 | 1.4-kb AccI fragment containing SHR3 in pRS202 | This work |
| pPL257 | GAP1∷FLU1 in pRS316 | Ljungdahl et al., 1992 |
| pPL288 | shr3Δ5∷hisG-URA3-neo-hisG in pBSII KS(+) | Kuehn et al., 1996 |
| pPL202 | 1.4-kb AccI fragment containing SHR3 in pBSII KS(+) | Ljungdahl et al., 1992 |
| pFG6 | 1.8-kb SalI–XmnI fragment containing SUC2 in pBSII KS(+) | This work |
| pFG8 | 1.8-kb SphI–PstI fragment containing SUC2 in pGEM-5Zf(+) | This work |
| pFG10 | 1.8-kb SphI–SalI fragment containing SUC2 in pRS316(ΔXN) | This work |
| pFG11 | pFG10 with NotI restriction site introduced into SUC2 | This work |
| pFG12 | SUC2-HA3 in pRS316(ΔNX) | This work |
| pFG19-pFG25 | 15-bp SphI–XbaI linker insertions in GAP1 in pPL247 | This work |
| pFG32-pFG38 | GAP1-SUC2 fusion constructs in pFG12 | This work |
| pFG40 | 6.4-kb SphI–SalI fragment containing suc2Δ100∷hisG-URA3-neo-hisG in pFG10 | This work |
| pFG80-pFG84 | gap1 mutant alleles with in-frame insertions encoding IEGRIEGR in pPL247 | This work |
| pFG117 | GST-SHR3 in pEGKT | This work |
| pFG118 | GST-CT-shr3(160–210) in pEGKT | This work |
| pFG119 | GST-shr3-23 in pEGKT | This work |
| pFG120 | GST-shr3ΔCT(163–201) in pEGKT | This work |
| pMB42 | 1.4-kb AccI fragment containing shr3-23 in pRS202 | This work |