Figure 1.
Dictyostelium expresses Dd14–3-3, a protein homologous to the 14–3-3. (A) Immunoblot analysis and biochemical localization of Dd14–3-3. Lane 1, vegetative Dictyostelium Ax2 cells; lane 2, 4-h developed Dictyostelium Ax2 cells; lane 3, recombinant 14–3-3ζ; lane 4, immunoprecipitation of Dd14–3-3 from developed Dictyostelium cells. (B) Biochemical localization of Dd14–3-3. Ax2 cells were developed and at the indicated times, 1 μM cAMP was added to 1 × 107 cells, which were lysed and fractionated into cytosol (C) and membrane (M) fractions as described in MATERIALS AND METHODS. Samples were subjected to 12% SDS-PAGE and blotted onto nitrocellulose. Immunoblots were stained with anti-14–3-3 antibodies.