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Inhibition of MHC-PKC activity by 14–3-3ζ. Various concentrations of recombinant 14–3-3ζ were incubated with MHC-PKC in a kinase assay as described in MATERIALS AND METHODS. The inhibition was expressed as a percentage of the kinase activity with the inhibitor as compared with the total kinase activity without the inhibitor. Data are the mean ± SEM (n = 4).
