Figure 3.

MHC-PKC forms a complex with Dd14–3-3 that is cAMP-dependent. (A) Ax2 cells were developed and stimulated with cAMP, and cell lysates were prepared as described in MATERIALS AND METHODS. Top, the cell lysates were immunoprecipitated with 14–3-3 antibodies and subjected to Western blot analysis using MHC-PKC antibodies as described in MATERIALS AND METHODS. Bottom, the cell lysates were immunoprecipitated with MHC-PKC antibodies and subjected to Western blot analysis using 14–3-3 antibodies as described in MATERIALS AND METHODS. (B) Quantification of the amounts of MHC-PKC and Dd14–3-3 in the complex shown in A and the activity of MHC-PKC that was measured as described in MATERIALS AND METHODS.