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MHC-PKC-C1 localized to the cytosol regardless of cAMP stimulation. MHC-PKC-C1 cells were developed, and at the indicated time points, 1 μM cAMP was added to 1 × 107 cells that were lysed and fractionated into cytosol (C) and membrane (M) fractions as described in MATERIALS AND METHODS. Samples were subjected to SDS-PAGE on 12% gels and blotted onto nitrocellulose. Immunoblots were stained with anti-MHC-PKC antibodies.
