Figure 5.

Rescue of the ida7 motility defects by transformation with constructs of the IC140 genomic region. (A) Diagram of the 11.5-kb wild-type genomic fragment containing the IC140 gene. Areas of interest based on the sequence analysis of Yang and Sale (1998) are shown here. The open rectangles indicate the exons of the IC140 gene, and the solid lines indicate the introns. The predicted translation start and stop sites are indicated, as well as the probable polyadenylation site. The regions containing TATA boxes and “tub” boxes are denoted by brackets. (B) Diagram of the IC140 gene constructs used to transform the ida7 mutant. The constructs are drawn with respect to the diagram of the IC140 gene above. The name of each plasmid construct is listed on the left, and the number of rescued transformants relative to the total number of transformants screened is presented as a ratio on the right.