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. 1998 Dec;9(12):3351–3365. doi: 10.1091/mbc.9.12.3351

Figure 6.

Figure 6

Expression of IC140 transcripts in wild-type, ida7, and rescued strains. Shown here are Northern blots of total RNA isolated before (0) or after (45) deflagellation. RNA (20 μg per lane) was separated on a 1% formaldehyde agarose gel, transferred to a Magnagraph membrane, and hybridized to a 1.6-kb cDNA fragment of the IC140 gene. A single 3.7-kb transcript is present in both the wild-type and AH1, and the expression of this transcript is up-regulated by deflagellation. The 3.7-kb transcript is completely missing in ida7. A smaller transcript is expressed at high levels both before and after deflagellation in the 5A strain. Hybridizing the blot with a control probe for the CRY1 gene, which encodes the S14 ribosomal protein subunit, confirmed that equal amounts of RNA were loaded in each lane (Perrone and Porter, unpublished results).