(A) The kinetic scheme used to model the triheteromeric receptors. Glutamate binding and unbinding to the NR2A and NR2B subunits was independent, thus there were two single-bound states (1A, 1B). Binding and unbinding rates for NR2A and NR2B were as in the previous models; all other rate constants were the geometric means of the values in the previous models (Table 2). (B) Opening and closing kinetics for our model triheteromeric NR2A/B-containing receptor. Open probability and decay kinetics were intermediate between the diheteromeric receptors, but more similar to those of NR2A-containing receptors. (C) Average number of each type of receptor present at the synapse, based on our simulations and the results of Nimchinsky et al. [26], under three different assumptions: that only diheteromeric NMDARs were present (AA, BB), that only NR2B- and NR2A/B-containing receptors were present (AB, BB) and that all three receptor types were present and combined randomly (AA, AB, BB). In all three cases, NR2B subunits made up greater than 85 percent of the subunit content at the synapse. There was no positive solution for the case where only NR2A and NR2A/B-containing receptors were present.