Figure 2. (A–D) Representative in situ hybridization autoradiograms of parasagittal sections of adult male zebra finches at the level of HVC (∼1.4 to 2.4 mm from the midline).
(A, left) Schematic depicting the major telencephalic song nuclei (in black), thalamorecipient areas (i.e. L2a, Bas), and major brain subdivisions. (A, center and right) Expression of known HVC markers. The other panels (B–D) depict representative autoradiograms of HVC markers that are exclusive to HVC (B), enriched in HVC and other song nuclei, such as LMAN (C, left), or area X (C, center and right; inset shows area X from a more medial section), enriched in 3/4 major song nuclei (D, left and middle), or are negative markers (D, right). Notice that some markers are also enriched in distinct pallial and striatal areas, and/or primary sensory areas. Scalebar: 1 mm. Gene abbreviations in Table S1. Anatomical abbreviations: A, Arcopallium; Bas, nucleus basorostralis; Cb, Cerebellum; GP, globus pallidus; Hp; Hippocampus; H, Hyperpallium; L2a, subfield L2a of field L; LMAN, lateral magnocellular nucleus of the anterior nidopallium; M, Mesopallium; N, Nidopallium; RA, robust nucleus of the arcopallium; Ot, optic tract; Rt, nucleus rotundus; St; striatum.